RNA

Part:BBa_K1442026:Design

Designed by: Yin Ho Vong   Group: iGEM14_Warwick   (2014-08-28)


siRNA target to human DPP-IV mRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design to ensure it was reverse-complemented and also complementary to a conserved sequence.


Source

Derived from reverse complement of a conserved sequence of DPP-IV mRNA

References